Research Article |
Corresponding author: Kaori Yokochi ( yokochi.kaori@gmail.com ) Academic editor: Jan Olof Helldin
© 2015 Kaori Yokochi, Roberta Bencini.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Yokochi K, Bencini R (2015) A remarkably quick habituation and high use of a rope bridge by an endangered marsupial, the western ringtail possum. In: Seiler A, Helldin J-O (Eds) Proceedings of IENE 2014 International Conference on Ecology and Transportation, Malmö, Sweden. Nature Conservation 11: 79–94. https://doi.org/10.3897/natureconservation.11.4385
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Rope bridges are being increasingly installed worldwide to mitigate the negative impacts of roads on arboreal animals. However, monitoring of these structures is still limited and an assessment of factors influencing the crossing behaviours is lacking. We monitored the use of a rope bridge near Busselton, Western Australia by the endangered western ringtail possums (Pseudocheirus occidentalis) in order to identify the patterns of use and factors influencing the crossings. We installed motion sensor cameras and microchip readers on the bridge to record the crossings made by individual animals, and analysed these crossing data using generalised linear models that included factors such as days since the installation of the bridge, breeding season, wind speed, minimum temperature and moonlight. Possums started investigating the bridge even before the installation was completed, and the first complete crossing was recorded only 36 days after the installation, which is remarkably sooner than arboreal species studied in other parts of Australia. The possums crossed the bridge increasingly over 270 days of monitoring at a much higher rate than we expected (8.87 ± 0.59 complete crossings per night). Possums crossed the bridge less on windy nights and warm nights probably due to the risk of being blown away and heat stress on warmer days. Crossings also decreased slightly on brighter nights probably due to the higher risk of predation. Breeding season did not influence the crossings. Pseudocheirus occidentalis habituated to the bridge very quickly, and our results demonstrate that rope bridges have a potential as an effective mitigation measure against the negative impacts of roads on this species. More studies and longer monitoring, as well as investigating whether crossing results in the restoration of gene flow are then needed in order to further assess the true conservation value of these crossing structures.
Road ecology, rope bridge, habitat fragmentation, Pseudocheirus occidentalis , wildlife crossing structures
Roads can act as a barrier to movement and gene flow in wildlife populations and cause genetic isolation and fragmentation. This can results in lowered fitness and adaptability, which increases the risk of population extinction (
Arboreal species can be especially affected by roads because of their fidelity to canopies and naivety on the ground (
In Western Australia a rope bridge was installed on Caves Road near Busselton in 2013. The bridge was targeted to provide safe crossing for the western ringtail possum (Pseudocheirus occidentalis), a nocturnal, folivorous, arboreal marsupial endemic to southwest Western Australia (Figure
Photographs of the western ringtail possum (Pesudocheirus occidentalis). a A possum at Locke Nature Reserve b A possum roadkill in Busselton, Western Australia.
We monitored the use of this bridge by P. occidentalis and other fauna to identify the patterns of use and factors influencing the crossings. In previous studies, animals have been observed to show reluctance towards wildlife crossing structures for a certain period of time before they habituated to them and started using them regularly (
Several arboreal marsupials increase their activity ranges or change their movement patterns during the breeding season in search of mates and additional resources (
In July 2013, a rope bridge was constructed across Caves Road about 9 km west of Busselton, Western Australia (33°39'32"S; 115°14'26"E) to connect peppermint trees in Locke Nature Reserve to those in a campsite across the road. Caves Road is a 15 m wide major road connecting popular tourist destinations in the region. The recorded daily traffic volume on this road was 6,000 cars in 2008, but it could vary up to 15,000 cars in the peak tourist season (
The rope bridge was supported by an approximately 8.5 m tall wooden pole with a concrete foundation and two metal stay wires on each side of the road. The bridge was 300 mm in width and approximately 26.5 m in length. Two steel wires running between poles with nettings of marine grade ropes in between provided a flat surface for possums to cross (Figure
A rope bridge installed on Caves Road near Busselton, Western Australia. a Two stay wires and a rope extending from the pole of a rope bridge to nearby trees on South side of Caves Road b Close up of the bridge showing one of the sensors and microchip reader on the North side (taken by an infrared camera on the bridge).
We captured 44 female and 53 male western ringtail possums within two 200 m x 200 m blocks on the North and South sides of the rope bridge site on Caves Road from March 2010 to April 2014 (Figure
A map of the study area near Busselton, Western Australia. Black rectangles represent the areas where Pseudocheirus occidentalis were captured for tagging, and the thick red line represents the rope bridge across Caves Road.
Thirty days after the installation of the rope bridge, an infrared camera (BuckEye Cam Orion camera, BuckEye Cam Australia, Victoria), a microchip reader (LID650N / ANT612 system, Dorset Identification B.V., Aalten, Netherlands), and a pair of optical sensors were set up on each end of the bridge. When an animal moved past and blocked one of the sensors, this triggered the camera to take three consecutive photos and activated the microchip reader for a period of 30 seconds. Date and time were recorded on every photograph taken, and the microchip readers recorded the date, time and microchip code of individuals that used the bridge. Unfortunately, the microchip readers malfunctioned regularly, so we used photographic data from 270 nights of monitoring from August 2013 to May 2014 for further analyses.
A crossing was regarded “partial” if an animal was recorded on one side of the bridge only and returned back to its original side. A crossing was regarded “complete” if an animal was recorded leaving one side and then arriving on the other side within 10 minutes. We recorded the simultaneous crossing by two and three adults as two and three crossings respectively; however, a crossing by a pair of mother and young was counted as a single crossing. Species, type, and direction of the crossings were obtained from photographic data to calculate the number of complete crossings of the bridge by P. occidentalis on each night.
We used generalised linear models with a negative binomial distribution and log link function to identify the factors influencing the number of crossings per night because the crossing data were discrete and overdispersed (
Candidate models used to analyse variables affecting the number of crossings of a rope bridge by Pseudocheirus occidentalis.
Variables | Hypothesis tested |
---|---|
Time | Crossings will increase over time. |
Breeding | Crossings will increase in breeding seasons. |
Min temp | Crossings will decrease on cold nights. |
Moon | Crossings will decrease on well lit nights. |
Wind | Crossings will decrease on windy nights. |
Time + Breeding | Crossings will increase over time and in breeding seasons. |
Time + Min temp | Crossings will increase over time but decrease on cold nights. |
Time + Moon | Crossings will increase over time but decrease on well lit nights. |
Time + Wind | Crossings will increase over time but decrease on windy nights. |
Min temp * Moon | Crossings will decrease on cold nights if the moon is bright. |
Null | The number of crossings varies randomly. |
We ran generalized linear models using the package MASS v.7.3-35 (
Within a week of installation of the poles, an author (KY) observed two western ringtail possums on stay wires investigating the poles. This was even before the metal wires and rope nettings were installed between the poles (i.e. before the installation of the bridge was completed). Three separate partial crossings by P. occidentalis were recorded on 16 photos on the first night of monitoring on the North end of the bridge. The first complete crossing from North to South was recorded on the 6th night of monitoring, only 36 days after the installation of the bridge had been completed. During 270 nights of monitoring, cameras recorded 664 complete crossings from North to South and 636 complete crossings from South to North, totalling 1300 crossings. The number of complete crossings increased gradually over time (Figure
Weekly averages of the number of complete crossings by Pseudocheirus occidentalis on a rope bridge installed over Caves Road near Busselton, Western Australia. The thick line shows the weekly averages and thin vertical lines represent standard errors of the means.
Microchip readers malfunctioned regularly, and not all possums using the bridge were microchipped, so only five microchipped individuals were recorded on eight nights. The North reader recorded one female partially crossing the bridge four times on one night, and the possum was also photographed on the bridge with her young on multiple occasions. After gaining independence, her young was recorded crossing the bridge on its own. Other mothers and their young as well as pairs of a male and a female were also regularly captured by the cameras while crossing the bridge together (Figure
Photographs of mother and young Pseudocheirus occidentalis crossing the road using the rope bridge near Busselton, Western Australia. The left photograph is of a mother and her young with another adult possum.
The number of crossings by P. occidentalis had a strong positive correlation with time since bridge installation (Table
Generalised linear model analysis on number of crossings of a rope bridge by Pseudocheirus occidentalis.
Variables included in the model | AIC | ∆AIC | Parameter estimates |
---|---|---|---|
Time + Wind speed | 1291.9 | Time = 0.007*, Wind = -0.011* | |
Time + Min temp | 1293.3 | 1.4 | Time = 0.007*, Min temp = -0.027* |
Time + Moon | 1296.8 | 4.9 | Time = 0.007*, Moon = -0.211* |
Time + Breeding | 1297.3 | 5.4 | Time = 0.007*, Breeding = -0.141 |
Time | 1299.1 | 7.2 | |
Wind | 1430.0 | 138.1 | |
Breeding | 1448.7 | 156.8 | |
Null | 1448.7 | 156.8 | |
Min temp | 1449.2 | 157.3 | |
Moon | 1449.6 | 157.7 | |
Moon * Min temp | 1451.4 | 159.5 |
As expected, the use of the rope bridge by P. occidentalis increased over time; however, the possums started crossing the bridge much sooner and at much higher rates than we expected. They started investigating the bridge even before the installation was completed, and the first complete crossing was recorded only 36 days after the installation, which is remarkably shorter than seven months – the shortest time elapsed prior to other possum and glider species starting to use rope bridges in other parts of Australia (
The rate of crossings was also considerably higher than those previously reported for other possums and gliders. Possums and gliders crossed the Pacific Highway in New South Wales using rope bridges at a rate of 0.02-0.08 crossings per night per species (
The number of bridge crossings decreased on windy nights, as expected. Being exposed to strong wind on the bridge may have discouraged possums from crossing due to the higher risk of being blown away. A higher risk of heat loss could be another reason for possums to cross the bridge less on windy nights (
The moonlight had a weak effect on the number of crossings, and fewer crossings were recorded on brighter nights. Whether this trend is caused by possums generally reducing their activities on bright nights or possums being discouraged to cross the exposed bridge on brighter nights cannot be known from our data.
Contrary to our expectation, the number of crossings did not increase during the breeding seasons. Home ranges of P. occidentalis in the same area also did not change during the breeding seasons (
Malfunction of the microchip readers made it impossible for us to identify all individuals using the bridge; however, the data still revealed that at least five different individuals used the bridge and that these individuals were using the bridge regularly. We must be cautious when interpreting the number of crossings on this bridge because a few individuals contributed to many of the crossings. At the same time, however, this also means that those individuals incorporated the bridge into their regular movement, which yet again suggests their high adaptability to this type of structure. To identify exactly how many individuals are benefitting from the bridge, we need to improve the monitoring system or develop a more reliable way of identifying individuals.
Multiple years of monitoring of the rope bridge in this study will also be necessary to investigate the long-term seasonal and yearly changes in the use of this bridge by P. occidentalis as well as to identify the asymptotic rate of crossing.
We also need to study the use of rope bridges in other areas in order to further assess the effectiveness of these structures as a wildlife crossing structure for P. occidentalis. Only one bridge was installed for this study due to financial constraints, and crossing patterns and characteristics are likely to differ in other areas with different population densities, habitats, and road characteristics or even for different kinds of artificial linear structures. For instance, it took P. occidentalis 18 months before it was recorded on another bridge installed across a newly constructed highway in Bunbury, located only 60 km away from the study area (B. Chambers, Pers. Comm.). This is possibly due to the lower density of the species in the area, recent disturbance caused by the road construction, and the greater length of the bridge (
Using individual based analyses such as parentage testing and Bayesian cluster analysis,
Roads pose negative impacts on wildlife and their impacts need to be mitigated by providing safe passages especially for threatened arboreal species. The critically endangered P. occidentalis habituated to a rope bridge remarkably quickly, and the bridge is now regularly used by multiple individuals at a high rate every night. This shows a high potential of rope bridges as an effective mitigation measure against the negative impacts of roads on this species. More studies and longer monitoring, as well as genetic investigations into whether crossing individuals are breeding across the road and resulting in the restoration of gene flow are needed in order to assess the true conservation value of these crossing structures.
The authors would like to thank Main Roads Western Australia, the Western Australian Department of Parks and Wildlife, Western Power, the School of Animal Biology at The University of Western Australia and the Satterley Property Group for technically and financially supporting this study. We also would like to acknowledge Mr Paul J. de Tores for his invaluable advice, support and training throughout the earlier stage of this study, and Dr Brian K. Chambers for his helpful advice and support in the analyses of data. We would also like to thank the City of Busselton, Abundant Life Centre, and Possum Centre Busselton Inc. for their support and over 100 volunteers, including Kaarissa Harring-Harris, who helped us in the field braving long hot, cold and/or wet days and nights. We thank the reviewer and Editor of this article for their constructive criticism of our manuscript.