Divergence time estimation by Bayesian inference tree of the 1069-bp cytochrome b gene sequences of Acheilognathus tabira tabira and outgroups. The blue rectangular bars on the nodes indicate the 95% highest probability density. Bayesian posterior probabilities are indicated at nodes, with values exceeding 0.90 shown. The node marked with an asterisk indicates the calibration point based on fossil record for the Acheilognathinae. Nodes with circled numbers are referenced in the text.