Research Article |
Corresponding author: Lucija Šerić Jelaska ( slucija@biol.pmf.hr ) Academic editor: Alessandro Campanaro
© 2017 Luka Katušić, Sven D. Jelaska, Lucija Šerić Jelaska.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Katušić L, Jelaska SD, Šerić Jelaska L (2017) Monitoring of saproxylic beetles in Croatia: following the path of the stag beetle. In: Campanaro A, Hardersen S, Sabbatini Peverieri G, Carpaneto GM (Eds) Monitoring of saproxylic beetles and other insects protected in the European Union. Nature Conservation 19: 39-56. https://doi.org/10.3897/natureconservation.19.12683
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As a member of the European Union, Croatia is obliged to report on the conservation status of 220 animal non-bird species listed in the annexes of the Habitats Directive (92/43/EEC), for which purpose a monitoring system is being established. Concerning saproxylic beetles, seven species present in its territory have to be monitored: Lucanus cervus, Cerambyx cerdo, Morimus funereus, Rhysodes sulcatus, Cucujus cinnaberinus, Rosalia alpina and Osmoderma eremita complex. Out of these species, a monitoring programme has only been established for Lucanus cervus, which partially includes participation of non-experts. In 2015 and 2016, a public campaign was organised in order to collect observations of Lucanus cervus and two other saproxylic beetles that are easily recognisable by the public: Morimus funereus and Rosalia alpina. Data gathered through this campaign serve as an addition to the mapping activities and monitoring of the species’ range. So far, more than 650 citizen observations have been collected, providing data on species presence in 216 10×10 km2 grid cells intended for reporting on the species’ range. Besides the public campaign, since 2014, public institutions for managing nature protected values have been involved in population monitoring for which they received education through several workshops. Altogether, 21 sites have been included in the monitoring of the stag beetle so far. Data collected for Lucanus cervus on standard transects, by tree and ground pitfall traps and tree trunk surveys at night will be discussed. To the present time, eight public institutions have been involved in stag beetle population monitoring and the number has been continuously increasing.
citizen science data (CSD), Lucanus cervus, Habitats Directive, habitat suitability model, monitoring, reporting, Morimus funereus, Rosalia alpina, species distribution range, observation on transects
As the most recent member of the European Union, the Republic of Croatia, joining in 2013, has an obligation, according to Article 17 of the Habitats Directive (92/43/EEC) and Article 12 of the Birds Directive (2009/147/EC), to report on the conservation status of the species and habitats listed in the annexes of the directives by 2019. The conservation status assessment has to be undertaken for seven saproxylic beetles, Lucanus cervus, Cerambyx cerdo, Morimus funereus, Rhysodes sulcatus, Cucujus cinnaberinus, Rosalia alpina and Osmoderma eremita complex which are present in its territory and listed in the annexes of the Habitats Directive.
Of the seven saproxylic beetles, to date a monitoring programme at the national level has only been developed for the stag beetle (
However, Croatia has its own specificities concerning geographical position, geology, forest management practice, as well as socio-economic and political history. The country spreads across three biogeographical regions: Mediterranean, Continental and Alpine and the stag beetle has existed in all three of them (
Before mapping and monitoring activities connected to the Habitats Directive, no other systematic surveillance for the stag beetle has been conducted in Croatia (
In the European Union, several non-invasive monitoring methods have been developed and tested over the last 20 years such as trapping of adults (with or without lures), counting living adults during transect walks (flying or on the ground), surveys of tree trunks, mapping adults by citizens, counting road kill individuals and predation remains etc. (e.g. Alvarez and Alvarez 1995, Campanaro et al. 2012 and
Due to a lack of experts, vis-à-vis the need for a large input when the Habitats Directive requirements are applied, it was decided to gradually include non-experts as far as possible in order to have financially feasible and scientifically relevant long-term surveillance of the stag beetle across three biogeographical regions. For the first time, the results have been presented on a systematic survey by non-experts from public institutions for management of nature protected values (national parks, nature parks, county institutions etc.) in monitoring stag beetle populations following the national monitoring programme; and unsystematic data gathering by citizens as a part of the surveillance that provided additional sources of data for mapping and monitoring of the range of the stag beetles. In addition, data gathering by citizens were organised for Morimus funereus and Rosalia alpine, that are among largest European saproxylic coleopterans and easily recognisable. After four years of surveillance, the efficiency of the stag beetle collecting methods and the potential of non-experts were discussed, as well as the problems which were faced during monitoring and mapping activities.
According to the Croatian national monitoring programme for the stag beetle, monitoring of the conservation status consists of (i) monitoring of population size and structure on plots, utilising three data sampling methods, counts on evening transects, survey of tree trunks and tree pitfall traps with lures; and of (ii) systematic range mapping, accompanied by non-systematic data gathering on the species distribution. The methodology was suggested as described below.
Monitoring on transects should be conducted along forest roads or walking paths inside the forest or at the forest edge during favourable weather conditions (e.g. without rain) in June/July period when it is the seasonal peak of adults’ activity (
The night survey of one to ten tree trunks should be conducted during warm and dry evenings, immediately after the evening transect. Tree trunks should be surveyed in ten successive days in two repetitions, preferably one in the second half of June and one in first half of July. This method was suggested by
The tree pitfall traps should be made of plastic bottles (2 litres) cut off to two thirds of their height, placing the upper part inside the lower part and thus making a plastic pot with a funnel. The opening should be broad enough for the stag beetle to fall into the trap. Three to ten traps should be placed per site, attached to a trunk of live standing tree, at 1.5–2 m of tree height. The traps should be filled with bait, e.g. fruit, rum with sugar or grated fresh ginger root (
The efficacy of these three sampling methods, proposed by the monitoring programme, was tested in the Continental region at two locations, Mt. Medvednica and Maksimir Park in Zagreb town, during June/July in 2013 and in the Mediterranean region, at Mt. Učka and Krk Island locations, in 2014, where the presence of the stag beetle had already been confirmed.
As mentioned above, due to the lack of experts, it was concluded that involvement of non-experts in monitoring the conservation status was required to fulfil the reporting obligations. As a part of the nature protection sector is already directly present in the field, employees of the public institutions (PIs) for management of nature protected values were recognised as prized field researchers who could facilitate the monitoring activities. In order to stimulate and educate these potential associates, in summer 2015, the Croatian Agency for the Environment and Nature Protection (CAEN), within the EU Natura 2000 Integration Project - NIP (IBRD 8021-HR), organised two workshops for the implementation of the monitoring of stag beetle population. The workshops consisted of theoretical and practical parts. During the theoretical part, participants were educated on saproxylic beetle ecological functions, threat status and conservation measures, stag beetle biology and finally on monitoring methodology. The practical part consisted of field visits to stag beetle habitats where all three methods were demonstrated and tested by the participants themselves. In total, 29 employees from 17 PIs participated at the workshops.
Besides population monitoring, all PIs were encouraged every season to record stag beetle observations in order to facilitate species mapping and range monitoring. For the same purpose, in spring 2015 and spring 2016, CAEN initiated a broad citizen science campaign for collecting observations of stag beetle and other two larger and easily recognisable species Morimus funereus and Rosalia alpina. The campaign was announced via the CAEN website, websites and mailing lists of several biological civil society organisations, most popular social networks and media. As well as data on observation locality and date, photographic evidence was requested for species identity verification. Citizen involvement was encouraged by rewarding every hundredth record and most attractive species photograph with the set of red books of wild species of Croatia. All reported observations were published and credited on the Bioportal - geoportal of the Croatian Nature Protection Information system (CAEN 2017).
As a result of previous actions aimed at encouraging PIs to participate in monitoring and mapping activities, eight PIs joined the monitoring activities within the next two years and conducted the monitoring on an additional six locations in the Continental biogeographical region.
At each location, one to two sites were selected (Table
Characteristics of study areas, PIs that were included and the year when monitoring was undertaken.
Biogeographical region | Location | Public Institution | No. of sites |
Year | Habitat type | Main tree species at location |
---|---|---|---|---|---|---|
Continental | Mt. Medvednica | Medvednica Nature Park | 1 | 2016 | Natural forest | Quercus petraea, Fagus sylvatica, Robinia pseudoacacia |
1 | 2015 | Natural forest | Quercus petraea, Fagus sylvatica, Robinia pseudoacacia | |||
2* | 2013 | Natural forest | Quercus petraea, Robinia pseudoacacia, Fagus sylvatica, Picea abies | |||
Zagreb | Maksimir Park | 2 | 2013 | Urban forest | Quercus petraea, Q. robur, Q. cerrris | |
Nedelišće | Međimurje Nature | 1 | 2016 | Forest edge | Quercus sp., Carpinus betula | |
Radoboj | Krapina-Zagorje County | 1 | 2016 | Quercus petraea | ||
Mt. Žumberak | Žumberak-Samoborskogorje Nature Park | 2 | 2016 | Quercus cerris, Fagus sylvatica, Carpinus betula, Populus alba, Acer pseudoplatnaus, Betula pendula, Prunus sp. | ||
Slavonski Brod, Mladavodica | Natura Slavonica, Brod-Posavina County | 1 | 2016 | Forest edge | Fagus sylvatica, Quercus petraea, Carpinus betula, Corylus avellana, Salix alba, Populus sp., Fraxinus sp. | |
Spačva | Vukovar-Srijem County | 2 | 2015 | Natural forest | Quercus robur, Carpinus betula | |
Spačva | Vukovar-Srijem County | 2 | 2014 | Natural forest | Quercus robur, Carpinus betula | |
Mt. Papuk | Papuk Nature Park | 2 | 2015 | Natural forest | Quercus petraea, Fagus sylvatica, Prunus sp. | |
Mediterranean | Mt. Učka* | Faculty of Science | 2 | 2014 | Forest edge | Quercus pubescent, Ostrya carpinifolia, Carpinus orientalis |
Krk Island* | Faculty of Science | 2 | 2014 | Natural forest | Quercus ilex, Q. pubescens, Carpinus orientalis |
Altogether, 21 transects at lengths between 100–700 m were set along forest roads or walking paths inside forests or at forest edges. The survey was conducted in the evenings between 7 pm and 9 pm by one person spending 30 minutes of slow continuous walking along the transect and counting flying adults and those on the ground. Evening transects were applied in successive 9 to 20 days when weather conditions were favourable. The relative abundance in Table
In addition, 4 to 12 traps were employed at each site and each trap was attached to the trunk of a live tree. The traps were filled with three different baits: fruit (peach and banana mixture), rum with sugar (only the first year,
Although it was not suggested by the national programme, ground pitfall traps were also used at three sites (Table
The results of simultaneous observations of stag beetles during 2013-2016 using three methods at 21 sites (and ground pitfall traps as an additional method at two locations in Spačva and Slavonski Brod area) in Croatia. Number of observations/individuals (No. obs. and No. ind.) and relative abundance for used methods at each location representing sampling effort are presented.
Location/Year | No. transects/sites (length, m) | Transect evenings | No. tree traps per site | No. ground traps per site | Traps exposure days | Evening transects | Tree traps | Ground traps | Night survey of trunks | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. obs | No.obs/ 100 m * day |
No. ind | No.ind/ one trap*day |
No. ind | No. ind/ one trap*day | No. ind | No.ind/ night*site | ||||||
Mt. Medvednica/2016 | 1 (700 m) | 9 | 10 | / | 19 | 13 | 0.21 | 7 | 0.037 | 0 | / | 0 | 0.000 |
Mt. Medvednica/2015 | 1 (700 m) | 9 | 10 | / | 17 | 19 | 0.30 | 54 | 0.318 | 0 | / | 0 | 0.000 |
Mt. Medvednica/2013 | 2 (100 m; 100 m) | 20 | 10 | / | 20 | 160 | 2.00 | 14 | 0.018 | 0 | / | 4 | 0.050 |
Zagreb, Maksimir Park/2013 | 2 (100 m; 100 m) | 20 | 10 | / | 20 | 122 | 1.53 | 2 | 0.003 | 0 | / | 4 | 0.050 |
Međimurje, Nedelišće/2016 | 1 (350 m) | 20 | 10 | / | 20 | 683 | 9.76 | 140 | 0.700 | 0 | / | 15 | 0.750 |
Zagorje, Radoboj/2016 | 1 (340 m) | 14 | 12 | / | 16 | 270 | 5.67 | 6 | 0.031 | 0 | / | 3 | 0.214 |
Mt. Žumberak/2016 | 2 (390 m; 900 m) | 11 | 4 | / | 17 | 39 | 0.14 | 0 | 0.000 | 0 | / | 25 | 0.568 |
SlavonskiBrod, Mladavodica/2016 | 1 (100 m) | 14 | 12 | 12 | 20 | 43 | 3.07 | 14 | 0.058 | 7 | 0.029 | 3 | 0.214 |
Spačva (Vukovar-Srijem County)/2015 | 2 (200 m; 200 m) | 19 | 12 | 12 | 19 | 137 | 1.14 | 19 | 0.021 | 0 | 0.000 | 20 | 0.333 |
Spačva (Vukovar-Srijem County)/2014 | 2 (200 m; 200 m) | 16 | 12 | 12 | 20 | 50 | 0.39 | 1 | 0.001 | 2 | 0.002 | 3 | 0.047 |
Mt. Papuk/2015 | 2 (100 m; 100 m) | 15 | 12 | / | 10 | 21 | 0.53 | 11 | 0.023 | 0 | / | 4 | 0.100 |
Mt. Učka/2014 | 2 (200 m; 200 m) | 17 | 12 | / | 22 | 119 | 0.88 | 12 | 0.011 | 0 | / | 7 | 0.103 |
Krk Island/2014 | 2 (200 m; 200 m) | 10 | 12 | / | 15 | 187 | 1.56 | 16 | 0.022 | 0 | / | 26 | 0.433 |
The survey of tree trunks was carried out between 8 p.m. and 9.30 p.m. in warm and dry nights. Ten to twenty trunks were randomly surveyed per site per day using an electric torch.
The relative abundance was calculated as the average number of individuals observed per surveyed trunk per day.
Monitoring of adult beetles was undertaken from 2013 to 2016 in period between 15 June and 19 July.
For comparisons of sampling data and methods efficiency, non-parametric statistical tests, χ² and Kruskal-Wallis were used. In addition, cumulative average number of individual species recorded per day at each transect was calculated to reveal how many consecutive days were needed before the average number of observed specimens per day stabilised on the transect (i.e. reduction of variation).
Spatial analyses of the results were made in ArcGIS 10.1. (ESRI 2012).
To estimate the efficiency of the citizen science campaign with respect to spatial coverage of data on the stag beetle, habitat suitability maps have been produced for the stag beetle using Maxent (
Overall, there were 2282 records of stag beetle adults after monitoring activities on 21 sites within ten locations, sampled during June/July from 2013 to 2016. Of the 21 sites, four were located within the Mediterranean biogeographical region and the rest in Continental Croatia. There was no systematic surveillance conducted in the Alpine region during this period. Such differences in the number of sites in each region were the consequence of the lower response from the public institutions of Alpine and Mediterranean regions to the workshops and monitoring activities.
Amongst three methods applied on all sites, the evening transect was the most efficient in detecting the species presence (Figure
Stag beetle detection efficiency using three sampling methods presented as a proportion of sites with recorded individuals (N sites = 21). The differences were not significant (χ² = 0.3658, p<0.05).
Furthermore, all tested methods were sex biased, with the detection of significantly more males than females (χ² = 13.0384, p-value =0.004554, p< 0.05). The lowest proportion of males, 80.7%, for the survey of trunks, 81.7% at counts on evening transects, 88.9% in ground pitfall traps and 90.2%.in tree pitfall traps was found.
Twenty days of evening transects, in two sequences of 10 days each as suggested by the monitoring programme, were achieved at five sites and observations at the other 16 sites were undertaken within 9 to 17 days. During that period, in eastern continental Croatia, in Spačva T1 2015, it can be clearly seen that, after 11 days, the average number stabilised at ca. 3.5 observed individuals on the transect and at the Mlade Vodice 2016 transect, the average number of individuals at ca. 3.5 was reached after 10 days of transect walk (Figure
Altogether 640 observations of the three saproxylic beetle species were reported to the CEAN by the public during the citizen science campaign, the majority being of stag beetle (555 observations, 87%), then 45 observation of Morimus funereus (7%) and 40 of Rosalia alpina (6%).
Observations were reported from all three biogeographical regions in Croatia, with the majority from the Continental biogeographical region (Figure
Observation of three saproxylic beetle species reported to the CEAN during the citizen science campaign.
Share of observations per species per biogeographical region in the total number of observations reported to CEAN during the citizen science campaign.
In total, citizen observations confirmed the presence of these three species in 216 10×10 km2 grid cells, intended for reporting on species’ range, out of which the presence of the stag beetle was confirmed in 204 grid cells (Figure
Number of 10×10 km2 grid cells with confirmed presence of three saproxylic beetle species, based on observation reported to the CEAN during the citizen science campaign.
Analyses of habitat suitability maps derived by Maxent using two sets of descriptors are shown in Figure
Overlap of Maxent habitat suitability derived maps based on human population orientated variables (Human) and those representing climatic and relief condition (TopoClim). A maps produced using the 0.5 threshold value for reclassifying Maxent output grids B maps produced using the custom threshold value for reclassifying Maxent output grids to include 80% of input data. (For more details, see Methods section).
The percentages of suitable habitats for the stag beetle identified by the Maxent model based on human population oriented variables, based on variables describing climatic and relief conditions and those where both models were congruent, are shown in Figure
Venn’s diagram of percentages of suitable habitats identified by Maxent derived models based on human population orientated variables (Human) and those describing climatic and relief conditions (ClimTopo). A areas obtained using the 0.5 threshold value for reclassifying Maxent output grids B areas obtained using the custom threshold value for reclassifying Maxent output grids to include 80% of input data. (For more details, see Methods section).
Of the three methods proposed by the Croatian stag beetle monitoring programme (
The lower detection efficiency of tree pitfall traps and trunk surveys and lower number of recorded individual species might raise the question of justification for their application. Trunk surveys proved to be more sensitive to females than the other two methods and pitfall traps in comparison with evening transects and trunk surveys can be set and checked during the working hours of PIs. Therefore, the pitfall trap method with appropriate bait can still be utilised when night surveys are not an option.
Collecting observations of the saproxylic beetle species through a citizen science campaign in Croatia proved that involvement of the public in mapping of these charismatic and easily recognisable species can make a considerable contribution to the knowledge of their distribution. The same conclusion is given by
Spatial analyses of data collected through citizen science campaign (Figure
Based on the four years’ experience in establishing a monitoring system for saproxylic beetles, it is concluded that, with appropriate education, non-experts can be successfully involved in population monitoring of these species, thus compensating for the lack of experts as well as reducing financial costs for the monitoring system. Although citizen science programmes, with accompanying media campaigns, can also considerably contribute to the mapping activities of easily recognisable and charismatic species, they cannot substitute targeted mapping projects due to the observation bias to urban areas.
LSJ and LK developed the initial concept of the paper. LSJ, LK and SDJ wrote the manuscript together. LSJ was involved in population monitoring activities and data analyses; LK was involved in contacting PIs, providing citizen science observations and data analyses and SDJ performed the spatial analyses of presented data and contributed to the initial concept of this paper.
We would like to thank all PIs and their employees and associates who have so far participated in the monitoring activities (K. Brkić, I. Ćosić, P. Dragičević, I. Gredičak, D. Hlavati, B. Horvatić, D. Hršak, M. Japundžić, D. Kranjčević, Z. Mandić, V. Markić, M. Pavičić, L. Pavičić, A. Petrović, M. Raguž, I. Rojko, N. Šaić, M. Ščavničar, P. Šemnički, N. Šoić, S. Šokčević, K. Vugrek Petljak, M. Zadravec) and all participants of our citizen science campaign. We also need to thank our colleagues D. Hlavati for managing the campaign and validating the reported observations, I. Špoljarić and B. Janev Hutinec for help with the workshops in Maksimir Park and National Park Plitvice Lakes and F. Kostanjšek, N. Jelaska, B. Jelaska, A. Kulić, J. Šerić, I. Rukavina and A. Smoković and for their great help in the field.
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