Research Article |
Corresponding author: Thomas Ziegler ( ziegler@koelnerzoo.de ) Academic editor: Franco Andreone
© 2018 Hai Ngoc Ngo, Truong Quang Nguyen, Tan Van Nguyen, Mona van Schingen, Thomas Ziegler.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Ngo HN, Nguyen TQ, Nguyen TV, van Schingen M, Ziegler T (2018) Microhabitat selection and communal nesting in the insular Psychedelic Rock Gecko, Cnemaspis psychedelica, in Southern Vietnam with updated information on trade. Nature Conservation 31: 1-16. https://doi.org/10.3897/natureconservation.31.28145
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The Psychedelic Rock Gecko, Cnemaspis psychedelica, was described in 2010 and certainly belongs to the most spectacular gecko discoveries worldwide. The species is endemic to two small offshore islands in Rach Gia Bay. Its striking colour pattern makes the species highly attractive for the international pet market. The existent Cnemaspis population is negatively affected by habitat degradation and predation by introduced macaques. We herein provide the first characterisation of microhabitat selection of this species, including seasonal variation on Hon Khoai and Hon Tuong islands, Ca Mau Province, Vietnam. We found that characteristics of the selected microhabitat, such as substrate type, temperature and canopy cover slightly differed between the wet and dry seasons. We also demonstrated age-related differences in the selection of perch heights. Communal nesting was, for the first time, reported for C. psychedelica, as well as natural predation by a snake species (Lycodon capucinus). In addition, we documented ongoing habitat destruction on Hon Khoai Island and recorded illegal trade of live Psychedelic Rock Geckos for the first time on local pet markets in both northern and southern Vietnam. Our findings highlight the need for improved conservation measures in order to reduce anthropogenic impacts on wild populations of C. psychedelica.
Microhabitat requirements, seasonal variation, communal nesting, natural predators, illegal trade, conservation
The old world gecko genus Cnemaspis Strauch is considered as one of the most species-rich paleotropical gekkonid genera, encompassing more than 100 species with a widespread distribution throughout tropical Africa, South Asia and Southeast Asia (
The known wild population of the species was estimated to comprise approximately 500 mature individuals (
The study provides detailed data on microhabitat selection of C. psychedelica within its natural habitat. We examined differences in microhabitat selections of lizards between the wet and dry seasons, as well as variations in habitat use amongst age classes. Furthermore, we provide new information about egg deposition behaviour, natural predation and ongoing habitat destruction on Hon Khoai Island, as well as new evidence for local trade in Cnemaspis psychedelica, highlighting the need for immediate measures to protect the remaining populations.
Study sites were selected based on known occurrences of C. psychedelica on Hon Khoai and on Hon Tuong islands, Ca Mau Province, southern Vietnam, according to previous surveys (
Mean annual air temperatures (°C) and monthly precipitation (mm) in 2015 and 2016 in Ca Mau Province, Vietnam (source: https://www.gso.gov.vn/default.aspx?tabid=713, accessed on 05 July 2018).
Field surveys were carried out during the wet season in November 2015 as well as during the beginning of the dry season in January 2016. Another short survey on Hon Khoai Island took place in January 2017. We mainly conducted night excursions after sunset between 19:00 h and 24:00 h. In order to determine the sex of the geckos and for taking measurements, individuals were captured by hand and subsequently released at the same spot in the morning or afternoon of the next day between 10:00 h and 17:00 h. While releasing geckos, further sighted individuals were also recorded, measured and released. Each gecko was measured with a digital slide-caliper to the nearest 0.1 mm. Abbreviations are as follows: snout-vent length (SVL), measured from tip of snout to anterior margin of cloaca; tail length (TL), measured from posterior of cloaca to tip of tail in geckos (
In order to investigate the biodiversity of Hon Khoai Island, we carried out respective field surveys in January 2016. During these surveys, a few amphibians and reptiles were also collected and subsequently deposited in the collection of the Institute of Ecology and Biological Resource (IEBR), Hanoi, Vietnam. Stomach contents of collected snakes were analysed after dissection in order to identify whether they might represent natural predators of C. psychedelica.
Microhabitat parameters were recorded for each sighted individual, including substrate type (classified as cliff, rock, branch, leaves, forest floor), perch height [in m] (vertical distance between captured animal and ground), percentage of vegetation or rock coverage above animal, position (resting outside, under a rock or within a crevice), substrate surface condition (dry or wet), activity (resting, feeding, foraging), air temperature [°C], substrate temperature [°C], animal’s body surface temperature [°C] and relative air humidity [%]. Air temperature and relative air humidity were measured with a digital thermo-hygrometer at a vertical height from the ground up to 2m height at each microsite (TFA Dostmann/Wertheim Kat.Nr.30.5015), while substrate and body surface temperatures of animals were determined with an infrared thermometer (Measupro IRT20).
To identify intraspecific differences in microhabitat selection of C. psychedelica, individuals were classified into different age classes according to
Chi-square tests and t-tests with P ≤ 0.05 were performed to determine significant differences in selected microhabitat parameters with categorical and continuous variables, respectively, between wet and dry season and amongst age classes. Statistical analyses were applied with the programme SPSS Version 16.0 (SPSS Inc., Chicago).
To obtain an overview on the availability of and evidence for trade in Cnemaspis psychedelica in Vietnam, we visited several local pet markets (one in Ca Mau Province where the species occurs, one in Dong Nai Province, one in Ho Chi Minh City and one in Ha Noi City) and further investigated different internet platforms, reptile Facebook pages and Forums. We also interviewed three local dealers offering the lizard online and two local keepers in March 2018, in order to obtain information on origins, commercial prices and networks of illegal trade in this species. Additionally, we conducted field surveys at previous survey sites located on Hon Khoai Island, Ca Mau Province in January 2017 to evaluate anthropogenic threats. Nearly 10 road workers on Hon Khoai Island were interviewed to determine the local use of the species. Names of interviewees were kept anonymous to ensure data privacy rights and internet links were not disclosed to prevent misuse.
A total of 569 sightings took place (156 during the wet season and 413 during the dry season) including both subpopulations from Hon Khoai and Hon Tuong islands, respectively. Cnemaspis psychedelica was found to be active in the surroundings of granite basements within thick forest coverage, dominated by semi-deciduous forest vegetation. Mean air temperatures were with 29.1 ± 0.75 °C (26.0–31.5 °C, n = 569) slightly higher than average substrate temperatures of 26.6 ± 1.07 °C (20.0–31.7 °C, n = 567). Regarding seasons, substrate temperatures were with 26.9 ± 0.77 °C (25.2–31.7 °C, n = 411) slightly higher during the dry season than temperatures during the wet season (26.1 ± 1.48 °C, 20.0–29.6 °C, n = 156) (t-Test, t = 6.23, df = 187.8, P < 0.001) (see Fig.
Microhabitat selection of Cnemaspis psychedelica A Substrate selection B Microsite selection C Box plots of substrate temperature D Box plots of canopy coverage E Box plots of perch height.
Environmental parameters characterising the microhabitat of Cnemaspis psychedelica.
Parameters | Wet season (Nov) | Dry season (Jan) | T-test |
---|---|---|---|
Canopy cover [%] | 0–100 (79.6±30.5) (n=156) | 0–100 (91.2±20.1) (n=413) | F=2.3; P=0.0004<0.05 |
Height [m] | 0.01–3 (0.75 ±0.39) (n=156) | 0.01–2.8 (0.7±0.33) (n=413) | F= 1.17; P-value= 0.22>0.05. |
Elevation [m] | 1–81 (32.0±19.9) (n=158) | 3–79 (35.6±17.8) (n=407) | |
Ambient Temp. [°C] | 26–31.5 (28.5±0.9) (n=158) | 23.8–30.3 (29.3±0.4) (n=413) | |
Substrate Temp. [°C] | 20–29.6 (26.1±1.5) (n=156) | 25.2–31.7 (26.9±0.8) (n=411) | F=2.6; P-value=0.0001<0.05 |
Relative air Humidity [%] | 71–100 (86.2±6.2) (n=158) | 65–80 (73.3±2.6) (n=413) | |
Monthly Precipitation (mm) (GSOV. 2016) (access 05 July 2018) | 272.4 | 0.2 | |
Mean monthly Temp. [°C] (GSOV. 2016) (access 05 July 2018) | 28.0 | 27.9 |
The Psychedelic Rock Gecko was found active during both the wet and dry seasons. Regarding daily activity pattern, C. psychedelica was observed active during any sighting between 10:00 h to 24:00 h. The vast majority of lizards were found in the shade, even if a patch of direct sunlight was in close proximity.
During the wet season, C. psychedelica was mainly found on loose granite rocks, followed by cliff and leaves on trees (61%, 38%, 1%, n = 413, respectively, see Fig.
We found adult specimens at mean heights of 0.76 m (n = 419), while juveniles commonly resided at lower heights of 0.61 m (n = 149) (t = 2.9, df = 296.4, P = 0.004). Both age classes preferred to occupy granite rock formations (60.8%, 74.2%, respectively), while another high percentage of adults resided on cliffs (29% vs. 9%, respectively) and a lower percentage of adults occupied branches (7.8%, 16.8%, respectively).
Egg depositions of Cnemaspis psychedelica happened on flat surfaces of cliff walls, without direct sunlight. Egg deposition sites were located under high vegetation coverage during both seasons (see Fig.
An ingested adult female of C. psychedelica (IEBR. 4141: SVL = 68.64 mm, TL = 74.8 mm) has been found within a preserved and subsequently dissected Common Wolf Snake (Lycodon capucinus, IEBR. 4211: SVL = 471.4 mm, TL = 95.11 mm) (Fig.
A Adult female Psychedelic Rock Gecko found as a prey item in the stomach of a Common Wolf Snake (Lycodon capucinus) B Wild-caught individual of Cnemaspis psychedelica offered for sale in a pet shop in Dong Nai Province, Southern Vietnam C, D Preserved reptile species in alcohol, used for traditional medicine on Hon Khoai Island.
During the survey in January 2017, the optimal habitats of C. psychedelica on Hon Khoai Island were found to have experienced further degradation due to expanding road construction and the building of further artificial ponds (see Fig.
A Forest degradation caused by building new roads B New artificial pond on Hon Khoai Island.
Trade in living C. psychedelica has been recorded by our team in local pet shops from northern Vietnam (Ha Noi City) and southern Vietnam (namely, Ca Mau Province where the species is distributed, further in Dong Nai Province and Ho Chi Minh City), as well as online in Vietnamese Reptile Forums, Zalo Online and Facebook. During our visit to two pet shops, one in Ca Mau Province and one in Dong Nai Province in March 2018, we observed 20 and only one remaining C. psychedelica, respectively, which were kept in small boxes (Fig.
During the survey in January 2017, we found some reptile species such as Cnemaspis psychedelica, Cyrtodactylus leegrismeri, Draco maculatus, Hemidactylus frenatus, Gehyra mutilata, Gekko gecko and Ahaetulla prasina soaked together in rice wine and used for traditional medicine by road workers on Hon Khoai Island (Figs
We found that selected microhabitat characteristics such as substrate type, temperature and canopy cover slightly differed between the wet and dry seasons. Our data showed that the species is more frequently found under granite rock formations during the wet season, probably to avoid heavy rains (10 of 11 survey days). During the dry season, geckos were observed in remarkable numbers outside of caves or rock formations. The species was found strongly associated with granitic rock formations and cliffs during all life stages. While C. psychedelica has never been found on the forest floor and only scarcely within the vegetation, clutches were also unexceptionally deposited on rock formations at heights of at least 0.3 m above the ground. We assume that vertical cliffs might provide shelter from ground-dwelling predators.
Furthermore, animals were found at spots which were more densely covered with canopy during the dry season than during the wet season, which could be their behaviour to avoid direct sun exposure. Accordingly, fewer animals were found along transects with only slight vegetation coverage during the wet than during the dry season. These findings suggest the dependence of C. psychedelica on the availability of shaded habitats. Egg depositions were also exclusively found on cliffs, which are facing away from the sun.
Our study revealed that the body surface temperature of C. psychedelica was positively correlated with the substrate temperature (rs = 0.51, P < 0.001, n = 567). Thus, as in other ectotherms, basic physiological functions of C. psychedelica, such as locomotion, growth and reproduction are determined by the environmental temperature. Since tropical lizards are considered to have narrow temperature optima and only few options for behavioural and physiological compensation, they are assumed to be, in particular, vulnerable to extinction by climate warming (
We frequently observed aggregated oviposition sites and the placement of fresh eggs on top of or close to previous oviposition sites. Communal nesting is defined as “non-incidental deposition of eggs at a shared nest cavity by two or more co-specifics” (
With a closer look on the spatial distribution of C. psychedelica, we found age related differences in perch heights, namely juveniles occurring at significantly lower heights than adults. Similar habitat divergences between juveniles and adult individuals have also been reported for Cat Ba Tiger Geckos and Crocodile Lizards in Vietnam (
Reptiles, including lizards are commonly prey of other vertebrates, such as mammals and reptiles (
Lizards with limited geographic distributions, such as Cnemaspis psychedelica, may be extremely vulnerable to local habitat loss or alteration (
We thank the directorates of the Forest Protection Department of Ca Mau Province for their strong cooperation, the support of field work and issuing relevant permits. We are grateful to T.Q. Le, T.H. Tran, P.H. Dang (IEBR, Hanoi), H.V. Le and B.T. Nguyen (FPD of Ca Mau Province), K.V. Nguyen from Wildlife at Risk (WAR, HCM City) and M.T. Nguyen, T.T. Nguyen (VNMN, Hanoi) for their support and assistance in the field. We thank Sandra Altherr (Munich) for sharing unpublished information with us. Thanks also to Emanuele Scanarini, Ulrich Schepp, Rudolf von May and Franco Andreone for the reviews of previous manuscript versions. Field works on Hon Khoai were supported by Cologne Zoo, the Rufford Foundation (Grant No. 18631-2), the Federal Ministry for the Environment, Nature Conservation and Nuclear Safety (BMU) and the Deutsche Gesellschaft für Herpetologie und Terrarienkunde (DGHT) - Zoologische Gesellschaft für Arten- und Populationsschutz e.V (ZGAP) (Project code: 3.Cnemaspis psychedelica.VNM.2016.DGHT-4703). This research was partially supported by the national project entitled “Study on the biodiversity of offshore limestone islands in Vietnam: Proposed solutions and models for conservation and sustainable development (Code: KC09.11/16-20)”. Cologne Zoo is partner of the World Association of Zoos and Aquariums (WAZA): Conservation projects 07011, 07012 (Herpetodiversity Research, Amphibian and Reptilian Breeding and Rescue Station).